Bacteria can use this secretion system to manipulate eukaryotic host cells and/or combat other bacteria that thrive in the same ecological niche (Ho et al., 2013 Gallegos-Monterrosa and Coulthurst 2021). The type VI secretion system (T6SS) is a discovered mechanism in Gram-negative bacteria (Jani and Cotter 2010 Unni et al., 2022) by which cells in close proximity to one another can interact via contact-dependent protein transport from a donor cell to a recipient cell (Alvarez-Martinez and Christie 2009 Hood et al., 2010). It is possible to conclude that T6SS, although not essential, may participate in bacterial colonization, either by accelerating the infection or by promoting other mechanisms involved in it. The results obtained indicated a significant decrease in the epiphytic and endophytic colonization of the mutant with respect to the wild strain. In addition, properties associated with endophytic colonization were analyzed, such as the formation of biofilms and the production of pectinase and cellulase enzymes. J49-hcp strain was inoculated on peanut plants to analyze its colonization capacity. J49 strain was obtained by interrupting one of the essential tss genes. J49 strain enabled us to identify almost all the tss genes, except for the tssE gene. Initially, an in silico analysis looking for genes related to T6SS in the genome of the Enterobacter sp. The aim of this study was to determine whether the T6SS of a native peanut phosphate solubilizing bacterium is involved in its colonization in this legume. Among them, those with endophytic colonization capacity have survival advantages. Within plant promoting bacteria, those with phosphate solubilizing activity constitute a group of great relevance to the rhizosphere as they provide phosphorus to plants. The presence of T6SS in beneficial endophytic bacterial population associated with different plants suggests that it plays an important role in its interaction with the eucaryotic partner. In mutualistic endophytic bacteria, the type VI secretion system (T6SS) is related to important functions, such as interbacterial competition, stress response, quorum sensing, biofilm formation, and symbiosis.
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